目的 深入了解无茎黄芪(Astragalus acaulis)根的化学成分。方法 采用羟丙基葡聚糖凝胶Sephadex LH-20、Rp-18、硅胶等多种柱色谱对样品进行分离纯化,运用理化性质和现代波谱技术对单体成分进行结构鉴定。结果 从无茎黄芪根甲醇提取物的乙酸乙酯和正丁醇部位分离纯化得到19个化合物,分别鉴定为:甘草素(1)、异甘草素(2)、isomucronvlatol(3)、丁香脂素-4-O-β-D-葡萄糖苷(4)、落叶松脂醇(5)、落叶松脂醇-4-O-β-D-葡萄糖苷(6)、5-甲氧基落叶松脂醇-4-O-β-D-葡萄糖苷(7)、对羟基苯甲醛(8)、山梨醇(9)、7-氧谷甾醇(10)、7β-羟基谷甾醇(11)、β-sitosteryl-D-glucoside-6'-palmitate(12)、3β-羟基-5α,8α-桥二氧麦角甾-6,22E-二烯(13)、2α,3β-二羟基齐墩果酸(14)、硬脂酸甲酯(15)、β-谷甾醇(16)、胡萝卜苷(17)、油酸(18)和反式油酸甲酯(19)。结论 化合物1~19均为首次从该植物中分离得到;无茎黄芪富含黄酮类、木脂素类、甾体类成分。
Abstract
OBJECTIVE To study the major chemical constituents of the roots of Astragalus acaulis. METHODS Compounds were isolated and purified by using silica gel, Sephadex LH-20, and Rp-18 chromatographic techniques. Their structures were determined based on various spectral analysis and physicochemical properties. RESULTS Ninteen compounds were obtained from ethyl acetate and n-butanol fractions derivated from the methanol extract of the roots of A. acaulis, and identified as liquiritigenin (1), isoliquiritigenin (2), isomucronulatol (3), syringaresinol-4-O-β-D-glucopyranoside (4), lariciresinol (5), lariciresinol-4-O-β-D - glucopyranoside (6), 5-methoxylariciresinol-4-O-β-D-glucopyranoside (7), p-hydroxybenzaldehyde (8), sorbitol (9), 7-oxositosterol (10), 7β-hydroxysitosterol (11), β-sitosteryl-D-glucoside-6'palmitate (12), 5α,8α-epidioxy-ergosta-6,22E-dien-3β-ol (13), 2α,3β-dihydroxyloleanolic acid (14), methyl stearate (15), β-sitosterol (16), daucosterol (17), oleic acid (18), and methyl elaidate (19). CONCLUSION All of the compounds reported in this study are isolated from the plant for the first time. A. acaulis is rich in flavonoids, lignans, and steroids.
关键词
无茎黄芪 /
根 /
黄酮 /
木质素 /
甾体
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Key words
Astragalus acaulis /
root /
flavonoid /
lignan /
steroid
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中图分类号：
R284.1
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参考文献
[1] Editorial Board of Flora of China, Chinese Academy of Sciences. Flora of China(中国植物志) . Vol 42. Beijing:Science Press, 1993:208-213.
[2] QIAN Z G, JIA X Y, DAI R, et al. Study on species diversity of medicinal plant, genus Astragalas in Yunnan . J Yunnan Coll Tradit Chin Med (云南中医学院学报), 1997, 20(1):4-8.
[3] Medicinal Materials Company of Yunnan. List of Yunnan Traditional Chinese Medicine Resources (云南中药资源名录) . Beijing:Science Press, 1993:212-215.
[4] WU S H, LI K, ZHANG H K. Chemical constituents of Huoxiang Zhengqi Liquid . Chin Tradit Herb Drugs (中草药), 2011, 42(11):2189-2192.
[5] YU H W, HUA J, QIN L, et al. Chemical constituents of Premna yunnanensis . Chin Tradit Pat Med (中成药), 2012, 34(2):300-303.
[6] WEN Y H. Study on the chemical constituents in Astragalus membranaceus Bunge var. mongolicus (Bunge) Hsiao . Shenyang:China Medical University, 2008.
[7] ZHU W M, YIN C F, WANG S, et al. Chemical constituents of Porana spectabilis Kurz . Nat Prod Res Dev (天然产物研究与开发), 2001, 13(5):1-4.
[8] PING Y D, ZHANG Y H, LI Y. Antitumor activity of alcohol extract from Murraya exotica L. . Chin Pharm J (中国药学杂志), 2017, 52(18):1585-1588.
[9] KUANG H X, WANG B, WU Q Q, et al. Chemical constituents from Gentianella acuta . Chin Tradit Pat Med (中成药), 2017, 39(12):2546-2550.
FAN C Q, ZHU X Z, ZHAN Z J, et al. Lignans from Saussurea conica and their NO production suppressing activity . Planta Med, 2006, 72(7):590-595.
ZHOU H Y, ZHANG H, LI S M. Chemical constituents from leaves of Phyllostachys pubescens I . China J Chin Mater Med (中国中药杂志), 2005, 30(24):1933-1934.
SCHNARR G W, VYAS D M, SZAREK W A. Carbon-13 nuclear magnetic resonance spectra of acyclic carbohydrate derivatives:alditols, 1,2-bis(phenylhydrazones), and dithioacetals . J Chem Soc Perkin Trans 1, 1979:496-503.
DELLA GRECA M, MONACO P, PREVITERA L. Stigmasterols from Typha latifolia . J Nat Prod, 1990, 53(6):1430-1435.
GONG H F, YANG A M, LIU J X, et al. Studies on chemical constituents of Oxytropis kansuensis . Chin Tradit Herb Drugs (中草药), 2010, 41(2):187-190.
WANG M Y, YANG L, TU Y Y. Studies on the chemical constituents from stem of Chirita longgangensis var. hongyao . China J Chin Mater Med (中国中药杂志), 2006, 31(4):307-310.
JIANG B, ZHAO Q S, PENG L Y, et al. Constituents from Thamnolia vermicularis . Acta Bot Yunnan (云南植物研究), 2002, 24(4):525-530.
ZHAO M, DUAN J A, HUANG W Z, et al. Current situation and analysis of medicinal plant resources of Astragalus in China . Chin Wild Plant Res (中国野生植物资源), 2000, 19(6):5-9.
SUN Y J, WANG J M, WANG X, et al. Research progress on biological activity of naturally derived tetrahydrofuranoid lignan compounds . Chin Tradit Pat Med (中成药), 2014, 36(10):2159-2162.
XIAO C J, QIU L, XU W, et al. Chemical constituents from roots of Astragalus englerianus and their anti-oxidative activities . Chin Tradit Herb Drugs (中草药), 2015, 46(1):22-27.
CHENG X, XIAO C J, SUN J Z, et al. Study on the chemical constituents of Astragalus balfourianus . J Dali Univ (大理学院学报), 2014, 13(2):3-5.
ZHANG Q Z, WU X J, LIU D, et al. Comparison of DNA fingerprinting and active compound content among different species of Radix Astragali . Chin Pharm J (中国药学杂志), 2005, 40(6):457-459.
TIAN X Y, HAN B Y, XIAO C J, et al. Chemical and biological studies of Astragalus ernestii H. F. Comber . Nat Prod Res Dev (天然产物研究与开发), 2014, 26(2):188-192+259.
TIAN X Y, LIU Y L, YANG Y E, et al. Pharmacognostical study on seven species from Astragalus genus in Yunnan . J Chin Med Mater (中药材), 2013, 36(8):1257-1262.
ZHAO M Z. Studies on the chemical constituents and biological activities of Astragalus yunnanensis and Isodon flavida . Dali:Dali College, 2015.
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脚注
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基金
国家自然科学基金项目资助(31170313);大理大学滇西特色药用植物与白族药开发利用创新团队项目资助(ZKLX2019106)
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